{"id":3004,"date":"2019-09-20T21:23:56","date_gmt":"2019-09-20T21:23:56","guid":{"rendered":"https:\/\/phoenixmicrdev.wpengine.com\/?p=3004"},"modified":"2026-04-02T17:51:03","modified_gmt":"2026-04-02T17:51:03","slug":"phoenix-micron-oct-tracks-individual-stem-cells-in-the-rat-retina","status":"publish","type":"post","link":"https:\/\/phoenixmicron.com\/fr\/phoenix-micron-oct-tracks-individual-stem-cells-in-the-rat-retina\/","title":{"rendered":"Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> OCT tracks individual stem cells in the rat retina"},"content":{"rendered":"<p>In the July edition of Nanomedicine journal, Chemla et al demonstrate a fascinating and novel way to label and track individual photoreceptor precursor cells migrating within the retina with fluorescence and gold nanoparticle tagging using the Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> and OCT. Many retinal diseases such as age-related macular degeneration and retinitis pigmentosa are characterized by photoreceptor layer deterioration with healthy photoreceptor-support layers. Despite the relative health of most of the layers of the retina, the photoreceptor degeneration leads to impaired vision. A promising therapy is transplanting photoreceptor-precursor cells into the retina and being able to track their location and survival is essential. Chemla et al developed a cell-tagging mechanism with gold nanoparticles that are visible with OCT and CT scans.<\/p>\n<figure id=\"attachment_3005\" aria-describedby=\"caption-attachment-3005\" style=\"width: 714px\" class=\"wp-caption alignright\"><a href=\"https:\/\/phoenixmicron.com\/wp-content\/uploads\/2019\/09\/Screen-Shot-2019-09-20-at-2.00.45-PM.png\"><img decoding=\"async\" class=\"wp-image-3005 size-full lazyload\" data-src=\"https:\/\/phoenixmicron.com\/wp-content\/uploads\/2019\/09\/Screen-Shot-2019-09-20-at-2.00.45-PM.png\" alt=\"GNP PRP images\" width=\"714\" height=\"236\" data-srcset=\"https:\/\/phoenixmicron.com\/wp-content\/uploads\/2019\/09\/Screen-Shot-2019-09-20-at-2.00.45-PM.png 714w, https:\/\/phoenixmicron.com\/wp-content\/uploads\/2019\/09\/Screen-Shot-2019-09-20-at-2.00.45-PM-300x99.png 300w\" data-sizes=\"(max-width: 714px) 100vw, 714px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 714px; --smush-placeholder-aspect-ratio: 714\/236;\" \/><\/a><figcaption id=\"caption-attachment-3005\" class=\"wp-caption-text\">Figure 1: In the July edition of Nanomedicine journal, Chemla et al demonstrate a fascinating and novel way to label and track individual photoreceptor precursor cells migrating within the retina with fluorescence and gold nanoparticle tagging using the Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> and OCT. Many retinal diseases such as age-related macular degeneration and retinitis pigmentosa are characterized by photoreceptor layer deterioration with healthy photoreceptor-support layers. Despite the relative health of most of the layers of the retina, the photoreceptor degeneration leads to impaired vision. A promising therapy is transplanting photoreceptor-precursor cells into the retina and being able to track their location and survival is essential. Chemla et al developed a cell-tagging mechanism with gold nanoparticles that are visible with OCT and CT scans.<\/figcaption><\/figure>\n<p>The most common tagging mechanism is with fluorescent proteins, but the fluorescence may not be visible with fundus imaging if the eye has cataracts, the tagged cells are beneath the retinal pigment epithelium, or if the fluorescence is too dim and diffuse. The Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> produces beautiful images of fluorescently tagged cells in clear eyes as demonstrated in Figures 1 and 2. In an OCT image, fluorescently tagged cells blend into the retina and cannot be definitively identified. Chemla et al elegantly circumvented these issues by tagging their photoreceptor precursor cells with green fluorescent protein (GFP) as well as gold nanoparticles. Since the gold nanoparticles are solid, they are visible in an OCT image which allows for precise and detailed imaging of the cells as they integrate into the retina. They are nontoxic and long lasting; this technique could revolutionize tracking cells in the retina.<\/p>\n<figure id=\"attachment_3006\" aria-describedby=\"caption-attachment-3006\" style=\"width: 296px\" class=\"wp-caption alignleft\"><a href=\"https:\/\/phoenixmicron.com\/wp-content\/uploads\/2019\/09\/Screen-Shot-2019-09-20-at-1.59.40-PM.png\"><img decoding=\"async\" class=\"wp-image-3006 size-medium lazyload\" data-src=\"https:\/\/phoenixmicron.com\/wp-content\/uploads\/2019\/09\/Screen-Shot-2019-09-20-at-1.59.40-PM-296x300.png\" alt=\"Tagged photoreeptors\" width=\"296\" height=\"300\" data-srcset=\"https:\/\/phoenixmicron.com\/wp-content\/uploads\/2019\/09\/Screen-Shot-2019-09-20-at-1.59.40-PM-296x300.png 296w, https:\/\/phoenixmicron.com\/wp-content\/uploads\/2019\/09\/Screen-Shot-2019-09-20-at-1.59.40-PM.png 720w\" data-sizes=\"(max-width: 296px) 100vw, 296px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 296px; --smush-placeholder-aspect-ratio: 296\/300;\" \/><\/a><figcaption id=\"caption-attachment-3006\" class=\"wp-caption-text\">Figure 2. \u201cIn vivo monitoring of transplanted green fluorescent protein and gold nanoparticle-labeled photoreceptor precursors in the intravitreal space at various time points post transplantation. Cells were transplanted in the intravitreal space of rats and imaged at various time points post transplantation. (A) Fundus fluorescence camera imaging revealed diffused cell clusters in the vitreal space, which persisted up to 30 days (scale bar: 0.5 mm). (B) Optical coherence tomography imaging of the same treated eye at days 1, 7 and 30 (scale bar: 100 \u03bcm) revealed gold nanoparticle-labeled cells (marked by arrows) up to day 30. (B\u2019) Zoom-in of the demarcated area in (B). (C) Micro-computed tomography 3D-reconstructed images of the same eye on days 1, 7 and 30 also show presence of labeled cells (red), up to day 30 (scale bar 20 mm). (C\u2019) shows magnified areas of the regions demarcated in panel (C).\u201d<\/figcaption><\/figure>\n<p>Chemla et al injected the gold nanoparticle-tagged photoreceptor precursor cells subretinally or intravitreally into the eyes of Long-Evans rats and tracked the cells over 30 days. With the subretinal injection, discrete GFP-labeled cell clusters were visible using the Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> fundus camera throughout the 30 days (Figure 1, column A). Interestingly, the Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> OCT showed incredible detail of the gold nanoparticle-labeled cells migrating from the injection point to the inner retinal layers (Figure 1, column B and B\u2019). Without the gold nanoparticle labeling, the migrating cells are not visible using OCT. After the intravitreal injection, Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> fundus photography revealed diffuse GFP signal throughout the vitreous (Figure 2, column A), while the Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> OCT showed clusters of gold nanoparticle-labeled cells in the vitreous just above the retina (Figure 2, column B and B\u2019).<\/p>\n<p>Chemla et al demonstrated a novel, powerful technique for labeling with gold nanoparticles and tracking cells longitudinally in the retina using the Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> OCT.<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>Citation: Chemla Y, Betzer O, Markus A, Farah N, Motiei M, Popovtzer R, Mandel Y. (2019). Gold nanoparticles for multimodal high-resolution imaging of transplanted cells for retinal replacement therapy. Nanomedicine 14(14):1857-1871.<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>In the July edition of Nanomedicine journal, Chemla et al demonstrate a fascinating and novel way to label and track individual photoreceptor precursor cells migrating within the retina with fluorescence and gold nanoparticle tagging using the Phoenix MICRON\u00ae and OCT. Many retinal diseases such as age-related macular degeneration and retinitis pigmentosa are characterized by photoreceptor [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"categories":[22,35,1],"tags":[81,90,91,42,59,89,23],"class_list":["post-3004","post","type-post","status-publish","format-standard","hentry","category-micron-iv","category-oct","category-uncategorized","tag-fa","tag-fluorescence","tag-gold-nanoparticle-tagging","tag-phoenix-micron","tag-phoenix-micron-oct","tag-photoreceptors","tag-retina"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Phoenix MICRON\u00ae OCT tracks individual stem cells in the rat retina - Phoenix-micron<\/title>\n<meta name=\"description\" content=\"track individual photoreceptor precursor cells migrating within the retina with fluorescence and gold nanoparticle tagging using the Phoenix MICRON and OCT.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/phoenixmicron.com\/fr\/phoenix-micron-oct-tracks-individual-stem-cells-in-the-rat-retina\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Phoenix MICRON\u00ae OCT tracks individual stem cells in the rat retina - 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