{"id":2912,"date":"2019-06-19T02:58:32","date_gmt":"2019-06-19T02:58:32","guid":{"rendered":"https:\/\/phoenixmicrdev.wpengine.com\/?p=2912"},"modified":"2026-04-02T17:51:03","modified_gmt":"2026-04-02T17:51:03","slug":"dry-amd-model-with-the-phoenix-micron-laser-injector","status":"publish","type":"post","link":"https:\/\/phoenixmicron.com\/fr\/dry-amd-model-with-the-phoenix-micron-laser-injector\/","title":{"rendered":"Developing a unique, novel dry AMD model with the Phoenix MICRON\u00ae<\/span> laser injector"},"content":{"rendered":"

The most common cause of blindness in developed societies, age-related macular degeneration (AMD) robs the victim of the crucially important focused macular vision. While still devastating, wet AMD has a robust mouse model and the treatment option of anti-vascular endothelial growth factor (VEGF) subretinal injections. The Phoenix MICRON\u00ae<\/span> laser allows precise delivery of 532 nm energy on the rodent retina to break Bruch\u2019s membrane and induce choroidal neovascularization (CNV) and is extensively used in wet AMD research. Dry AMD, however, does not have a robust mouse model or treatment. Ibbett et al, in their paper, \u201cA lasered mouse model of retinal degeneration displays progressive outer retinal pathology providing insights into early geographic atrophy,\u201d ,\u201d use the Phoenix MICRON\u00ae<\/span> laser injector with their own 810 laser to develop an elegant mouse model that mimics the human dry AMD symptoms and will be crucial in the search for treatment.<\/p>\n

\"experimental
Figure 1: Experimental design with color fundus photography (CFP). The fundus images show the progressing of the laser burn from punctate areas (2) to a general atrophied area (5 and 6).<\/figcaption><\/figure>\n

The Phoenix MICRON\u00ae<\/span> provided clear color images of the longitudinal development of the laser burns from distinct spots to a merged geographic atrophy area very similar to the human dry AMD fundus (Figure 1). Using the contralateral eye as a control, the Phoenix MICRON\u00ae<\/span> focal ERG revealed that the lasered eyes had decreased function as shown by decreased amplitude a- and b-waves (Figure 2). OCT scans, histology, and electron microscopy all confirmed similarity to the human condition.<\/p>\n

\"Focal
Figure 2: focal ERG of lasered (grey trace) and control (black trace) eyes. A: Baseline ERG. B: 4 weeks after laser treatment. There was a significant decrease of a-wave and b-wave amplitudes in the lasered eyes.<\/figcaption><\/figure>\n

Ibbet et al used low power, high duration burns at 32 mW for 60 seconds to create the geographic atrophy. The laser burns morphed from distinct puncta to a merged atrophic area. Importantly, there were no signs of the CNV that is the hallmark of wet AMD (Figure 3). Several retinal layers degenerated to the point of being undetectable: the outer nuclear layer and inner\/outer segment layers were not visible in OCT or histology, and the outer plexiform layer and retinal pigment epithelium and Bruch\u2019s membrane morphology was altered. Histology revealed that the photoreceptors were destroyed in the lasered areas and that there was a distinct gradient from damaged to undamaged retinal areas. In the live fundus imaging, OCT, ERG, histology, and electron microscopy, the laser mouse model was remarkably similar to the human dry AMD condition.<\/p>\n

\"Fluorescein
Figure 3: Fluorescein angiography captured by the Phoenix MICRON\u00ae<\/span>. A: Healthy mouse. B: 8 weeks after laser treatment. C-D: Fluorescein angiography and corresponding bright field image (filters flipped from fluorescein settings to bright field). No leakage but hyperfluorescent ring around the lesions.<\/figcaption><\/figure>\n

Prior to this research, dry AMD rodent models included isolated features of dry AMD from genetically modified animals who had to be aged a very long time. Expensive and poor representations of the whole disease, these models were not satisfactory for a comprehensive study of dry AMD. In their thorough, molecule-to-mouse paper, Ibbett et al demonstrate an acute, consistent, and inducible rodent model of dry AMD, which is desperately needed for studying the pathology and potential treatments for the debilitating disease.<\/p>\n","protected":false},"excerpt":{"rendered":"

The most common cause of blindness in developed societies, age-related macular degeneration (AMD) robs the victim of the crucially important focused macular vision. While still devastating, wet AMD has a robust mouse model and the treatment option of anti-vascular endothelial growth factor (VEGF) subretinal injections. The Phoenix MICRON\u00ae laser allows precise delivery of 532 nm […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"categories":[73,74,22,1],"tags":[62,63,79,65,34,42],"class_list":["post-2912","post","type-post","status-publish","format-standard","hentry","category-cnv","category-laser","category-micron-iv","category-uncategorized","tag-amd","tag-cnv","tag-dry-amd","tag-laser","tag-mouse-model","tag-phoenix-micron"],"acf":[],"yoast_head":"\nDeveloping a unique, novel dry AMD model with the Phoenix MICRON\u00ae laser injector - Phoenix-micron<\/title>\n<meta name=\"description\" content=\"Learn about a new mouse model developed for dry AMD, the article and researchers who developed it using the Phoenix MICRON CMV System.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/phoenixmicron.com\/fr\/dry-amd-model-with-the-phoenix-micron-laser-injector\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Developing a unique, novel dry AMD model with the Phoenix MICRON\u00ae laser injector - 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