{"id":3669,"date":"2020-07-21T23:55:22","date_gmt":"2020-07-21T23:55:22","guid":{"rendered":"https:\/\/phoenixmicrdev.wpengine.com\/?p=3669"},"modified":"2026-04-02T17:50:43","modified_gmt":"2026-04-02T17:50:43","slug":"caspase-9-inhibiting-eyedrops-rescue-physiological-and-functional-retinal-vein-occlusion-damage-shown-with-phoenix-micron-oct-and-focal-erg","status":"publish","type":"post","link":"https:\/\/phoenixmicron.com\/es\/caspase-9-inhibiting-eyedrops-rescue-physiological-and-functional-retinal-vein-occlusion-damage-shown-with-phoenix-micron-oct-and-focal-erg\/","title":{"rendered":"Caspase-9 inhibiting eyedrops rescue physiological and functional retinal vein occlusion damage shown with Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span>, OCT, and focal ERG"},"content":{"rendered":"<p>In a recent well written, compelling article published in Nature Communications, \u201cEndothelial activation of caspase-9 promotes neurovascular injury in retinal vein occlusion,\u201d Avrutsky et al show that caspase-9 inhibition is a promising treatment for retinal vein occlusion. Retinal vein occlusion models hypoxic-ischemic neurovascular damage and is the second leading cause of blindness in working-age adults. Currently, treatment consists of anti-VEGF drugs which lead to inconsistent physiological and functional recovery. As caspase-9 expression is increased with hypoxic-ischemia, Avrutsky et al used caspase-9 inhibiting eyedrops, Pen1-XBir3, after retinal vein occlusion in mice.<\/p>\n<p><a href=\"https:\/\/phoenixmicron.com\/wp-content\/uploads\/2020\/07\/Screen-Shot-2020-07-21-at-4.43.56-PM.png\"><img decoding=\"async\" width=\"797\" height=\"612\" class=\"size-full wp-image-3670 lazyload\" data-src=\"https:\/\/phoenixmicron.com\/wp-content\/uploads\/2020\/07\/Screen-Shot-2020-07-21-at-4.43.56-PM.png\" alt=\"Figure 1. Phoenix MICRON&lt;span class=\" data-srcset=\"https:\/\/phoenixmicron.com\/wp-content\/uploads\/2020\/07\/Screen-Shot-2020-07-21-at-4.43.56-PM.png 797w, https:\/\/phoenixmicron.com\/wp-content\/uploads\/2020\/07\/Screen-Shot-2020-07-21-at-4.43.56-PM-300x230.png 300w, https:\/\/phoenixmicron.com\/wp-content\/uploads\/2020\/07\/Screen-Shot-2020-07-21-at-4.43.56-PM-768x590.png 768w\" data-sizes=\"(max-width: 797px) 100vw, 797px\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 797px; --smush-placeholder-aspect-ratio: 797\/612;\" \/><\/a><\/p>\n<p>The Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> image-guided laser was used to create retinal vein occlusion by targeting and ablating the major retinal veins of the mouse eyes (Fig 1B). Directly after and one day post-the laser injury, mice received the caspase-9 inhibitor Pen1-XBir3 or a sham eyedrop treatment. The Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> OCT revealed the subsequent retinal detachment, swelling, and disorganization of retinal inner layers after retinal vein occlusion (Fig 1B) as well as the presence of hyper-reflective foci\u2014though there is debate about what the foci are, they are a measure of retinal inflammation and the degree of vision recovery after retinal vein occlusion in humans. Insight 2D, a semi-automated segmentation program included with the Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> OCT, was used to measure the thickness of the retinal layers before and after retinal vein occlusion. Pen1-XBir3 treatment reduced the retinal swelling, accumulation of retinal fluid, and retinal detachment, preserving photoreceptor health (Fig 1C).<\/p>\n<p><a href=\"https:\/\/phoenixmicron.com\/wp-content\/uploads\/2020\/07\/Screen-Shot-2020-07-21-at-4.48.24-PM.png\"><img decoding=\"async\" width=\"220\" height=\"244\" class=\"wp-image-3671 size-full lazyload\" data-src=\"https:\/\/phoenixmicron.com\/wp-content\/uploads\/2020\/07\/Screen-Shot-2020-07-21-at-4.48.24-PM.png\" alt=\"Figure 2: Phoenix MICRON&lt;span class=\" src=\"data:image\/svg+xml;base64,PHN2ZyB3aWR0aD0iMSIgaGVpZ2h0PSIxIiB4bWxucz0iaHR0cDovL3d3dy53My5vcmcvMjAwMC9zdmciPjwvc3ZnPg==\" style=\"--smush-placeholder-width: 220px; --smush-placeholder-aspect-ratio: 220\/244;\" \/><\/a><\/p>\n<p>One, two and eight days after injury, the mice were injected with fluorescein and examined with the Phoenix MICRON to measure retinal blood vessel leakage, which Pen1-XBir3 significantly reduced (Fig 1B and 1C). To assess functional damage and recovery with treatment, the Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> focal ERG, with which the location of a focal white light stimulus is chosen under dark-red illumination to preserve dark adaptation, revealed that while retinal vein occlusion decreases b-wave amplitude (and to a lesser extent, a-wave amplitude), the Pen1-XBir3 treatment recovers the b-wave function (Fig 2).<\/p>\n<p>Using most of the suite of Phoenix MICRON<span class=\"reg-mark half-size\">\u00ae<\/span> products, Avrutsky et al demonstrated a powerful treatment for retinal vein occlusion that preserves the structure and function of the retina after intense vascular damage.<\/p>\n<p>&nbsp;<\/p>\n<p><em>Avrutsky, M. I., Ortiz, C. C., Johnson, K. V., Potenski, A. M., Chen, C. W., Lawson, J. M., White, A. J., Yuen, S. K., Morales, F. N., Canepa, E., Snipas, S., Salvesen, G. S., Jean, Y. Y., &amp; Troy, C. M. (2020). Endothelial activation of caspase-9 promotes neurovascular injury in retinal vein occlusion. Nature Communications, 11(1), 3173<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>In a recent well written, compelling article published in Nature Communications, \u201cEndothelial activation of caspase-9 promotes neurovascular injury in retinal vein occlusion,\u201d Avrutsky et al show that caspase-9 inhibition is a promising treatment for retinal vein occlusion. Retinal vein occlusion models hypoxic-ischemic neurovascular damage and is the second leading cause of blindness in working-age adults. [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_themeisle_gutenberg_block_has_review":false,"footnotes":""},"categories":[108,92,49,22,35,1],"tags":[113,117,80,119,120,121,23,118],"class_list":["post-3669","post","type-post","status-publish","format-standard","hentry","category-focal-erg","category-fluorescein-angiography","category-insight","category-micron-iv","category-oct","category-uncategorized","tag-anti-vegf","tag-caspase-9","tag-fluorescein-angiography","tag-mice","tag-mouse","tag-nature-communications","tag-retina","tag-retinal-vein-occlusion"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Caspase-9 inhibiting eyedrops rescue physiological and functional retinal vein occlusion damage shown with Phoenix MICRON\u00ae, OCT, and focal ERG - Phoenix-micron<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/phoenixmicron.com\/es\/caspase-9-inhibiting-eyedrops-rescue-physiological-and-functional-retinal-vein-occlusion-damage-shown-with-phoenix-micron-oct-and-focal-erg\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Caspase-9 inhibiting eyedrops rescue physiological and functional retinal vein occlusion damage shown with Phoenix MICRON\u00ae, OCT, and focal ERG - Phoenix-micron\" \/>\n<meta property=\"og:description\" content=\"In a recent well written, compelling article published in Nature Communications, \u201cEndothelial activation of caspase-9 promotes neurovascular injury in retinal vein occlusion,\u201d Avrutsky et al show that caspase-9 inhibition is a promising treatment for retinal vein occlusion. Retinal vein occlusion models hypoxic-ischemic neurovascular damage and is the second leading cause of blindness in working-age adults. 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Retinal vein occlusion models hypoxic-ischemic neurovascular damage and is the second leading cause of blindness in working-age adults. 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